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Details for Phage Doofinshmirtz
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Detailed Information for Phage Doofinshmirtz
Discovery Information
Found ByJennifer Joo, William Angel
Year Found2013
Location FoundCentreville, VA United States of America
Finding InstitutionUniversity of Maryland, Baltimore County
Program Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
GPS Coordinates38.83198 N, 77.4644 W Map
Discovery NotesThe phage was isolated from a soil sample taken at a depth of 10.6 cm from a vegetable garden behind a person's house on 09/07/13 at 7:45PM. The approximate air temperature was 75°F (23.9°C). The soil felt dry, but was not bone-dry as it had a slight bit of moisture. The soil was loose, soft, and reddish and did not contain many rocks or other debris. The house was surrounded by forest and was near a major road (U.S. Route 29/Lee Highway). The garden had plots of corn, cabbage, turnips, and lettuce.
Naming NotesNamed after a character from the show "Phineas and Ferb": Dr. Heinz Doofenshmirtz, a mad scientist. The name was changed by a single letter to avoid copyright infringement.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 10, 2014
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3327
Genome length (bp)161793
GC Content38.7%
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length2818 bp
End Determination NotesBuildup of reads from Illumina data.
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Other Cluster Members
Plaque NotesThe plaques of our phage were 2.5 to 3.0 mm in diameter and clear, resembling fireballs in shape, with one rounded side and one jagged side.
MorphotypeMyoviridae
Final annotation complete?No
Has been Phamerated?No
Uploaded to GenBank?No
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload