Details for Phage Limpius21
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| Detailed Information for Phage Limpius21 | |
| Discovery Information | |
| Found By | Alexis (Lexi) N. Robinson |
| Year Found | 2015 |
| Location Found | Fredericksburg, VA United States |
| Finding Institution | University of Mary Washington |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| GPS Coordinates | 38.293611 N, 77.4625 W Map |
| Discovery Notes | This soil sample was collected on August 24, 2015 at 9:35 am from a garden outside an apartment in downtown Fredericksburg, Virginia. The forecast that day was clear with a temperature of 24°C, with no rain or extreme weather conditions earlier that week. The garden in which the soil sample was extracted from is kept by paid landscapers, and the garden itself is roughly two feet away from the entrance of an apartment building: with a plethora of shrubs and small trees utilizing its resources. |
| Naming Notes | The phage name derives from the first letter of my nickname (L), followed by the root "imp" to reflect the bacteriophage's difficult physiology and plaque cultivation. The numerical value, 21, is the age I am turning at the end of the semester in which I am working with the phage, marking the conclusion of it's initial "discovery". A milestone, in essence. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Plaque Notes | Initial cultivation of the phage gave way to two plaque types: large, temperate plaques with a cloudy center and clear defined outer ring (2mm)and small, lytic plaques with a clear center and cloudy outer ring (0.5mm) Within each plating sequence, the phage cultivated plaques in colonies of 1-4. Host infection and phage cultivated consistently presented the smaller subspecies: 0.5mm-1mm in size with a clear center and cloudy outer ring. Throughout the duration of this study, plaque morphology gradually changed: the interior of the plaques cloudy over time--suggesting the temperate bacteriophage transition to the lytic infection cyclic periodically. |
| Final annotation complete? | No |
| Has been Phamerated? | No |
| Uploaded to GenBank? | No |