Details for Phage Nigalana
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Detailed Information for Phage Nigalana
Discovery Information
Found ByNavpreet Saini
Year Found2013
Location FoundManassas, VA United States of America
Finding InstitutionVirginial Commonwealth University
Program Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
GPS Coordinates38.742153 N, 77.426758 W Map
Discovery NotesThe BTK bacteriophage "Nigalana" was isolated from a soil sample taken from a rural area in Manassas on 9/3/13. The area was known for having pasture animals and the temperature was around 21°C. The dirt sample was enriched in the VCU phage discovery lab.
Naming NotesThe name Nigalana was given to the bacteriophage because "Nigalana" by definition in Punjabi means "to devour" and in Greek "Phagein" means "to devour".
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 22, 2014
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454
Approximate Shotgun Coverage96
Sanger Finishing Reactions33
Genome length (bp)163041
GC Content38.7%
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length2867 bp
End Determination NotesBuildups of reads in the 454 sequencing data.
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Other Cluster Members
Plaque NotesThe plates were shown to have clear plaque spots and they contained no halos. Each plaque had an average diameter of 2 mm.
MorphotypeMyoviridae
Final annotation complete?No
Has been Phamerated?No
Uploaded to GenBank?Yes: KU737344.1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload