Details for Phage NotTheCreek
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Detailed Information for Phage NotTheCreek
Discovery Information
Found ByNatasha Smith
Year Found2013
Location FoundSouth Brunswick, NJ United States
Finding InstitutionVirginial Commonwealth University
Program Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
GPS Coordinates40.399106 N, 74.51575 W Map
Discovery NotesThe phage was isolated from soil found at the bottom of a 6 in hole in the front yard of a house in a residential neighborhood. It was approximately 24 degrees Celsius.
Naming NotesI was originally trying to isolate a phage from a soil sample at the edge of a creek, but wasn't able to. "NotTheCreek" is short for "not from the creek."
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 5, 2014
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454
Approximate Shotgun Coverage100
Sanger Finishing Reactions6
Genome length (bp)159336
GC Content38.2%
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length2585 bp
End Determination NotesBy doubling of read coverage in Consed.
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Other Cluster Members
Plaque NotesTwo consistent plaque morphologies are present, 1mm in diameter and .6mm respectively. Plaques are clear and without halos.
MorphotypeMyoviridae
Final annotation complete?No
Has been Phamerated?No
Uploaded to GenBank?Yes: KU737351.1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload