| Detailed Information for Phage NotTheCreek | |
| Discovery Information | |
| Found By | Natasha Smith |
| Year Found | 2013 |
| Location Found | South Brunswick, NJ United States |
| Finding Institution | Virginia Commonwealth University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| GPS Coordinates | 40.399106 N, 74.51575 W Map |
| Discovery Notes | The phage was isolated from soil found at the bottom of a 6 in hole in the front yard of a house in a residential neighborhood. It was approximately 24 degrees Celsius. |
| Naming Notes | I was originally trying to isolate a phage from a soil sample at the edge of a creek, but wasn't able to. "NotTheCreek" is short for "not from the creek." |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 5, 2014 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Approximate Shotgun Coverage | 100 |
| Sanger Finishing Reactions | 6 |
| Genome length (bp) | 159336 |
| GC Content | 38.2% |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 2585 bp |
| End Determination Notes | By doubling of read coverage in Consed. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | C |
| Subcluster | C1 |
| Other Cluster Members |
Click to ViewHydralpha (None) JoeB (None) AaronPhadgers (C1) ALPS (C1) Archie14 (C1) Azalea (C1) Belinda (C1) Bhoosica (C1) Cletus (C1) DingnCase (C1) Dingus (C1) DirtyBetty (C1) Doofinshmirtz (C1) Elmer (C1) Escavirius (C1) Eyuki (C1) Freefood (C1) FreightTrain (C1) Grumio (C1) Hakuna (C1) Helga (C1) HonestAbe (C1) Ilona (C1) Kida (C1) Kota (C1) Lilihipha (C1) Megatron (C1) MightyMouse (C1) Momo (C1) Nemo (C1) Nigalana (C1) NotTheCreek (C1) OmnioDeoprimus (C1) Parsons (C1) PPIsBest (C1) Rex16 (C1) SageFayge (C1) SalinJah (C1) STSebastian (C1) Terra (C1) WPh (C1) Zuko (C1) Anthony (C2) Bastille (C2) CAM003 (C2) Evoli (C2) Herbert (C2) HoodyT (C2) Hudslayer (C2) Janet (C2) Kamfam (C2) OTooleKemple52 (C2) Vinny (C2) Adelynn (C3) Ampersand (C3) Anthos (C3) AvesoBmore (C3) B4 (C3) BigBertha (C3) ChannelFever (C3) Chotacabras (C3) ChurroBlast (C3) Flapjack (C3) GypsyDanger (C3) Joonam (C3) Juglone (C3) Natp (C3) Oregano (C3) Phabio (C3) Phrodo (C3) Riley (C3) Spock (C3) Taffo16 (C3) Troll (C3) Typhen (C3) BC4U (C4) BCP78 (C4) TsarBomba (C4) Bcp1 (C5) BM10 (C5) Hobo (C5) Kioshi (C5) |
| Plaque Notes | Two consistent plaque morphologies are present, 1mm in diameter and .6mm respectively. Plaques are clear and without halos. |
| Morphotype | Myoviridae |
| Final annotation complete? | Yes |
| Number of Genes | 296 |
| Has been Phamerated? | No |
| Uploaded to GenBank? | Yes: KU737351.1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
