Details for Phage Phrodo
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Detailed Information for Phage Phrodo
Discovery Information
Found ByShelby Edling
Year Found2014
Location FoundBelfair, WA United States of America
Finding InstitutionVirginial Commonwealth University
Program Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
GPS Coordinates47.46957 N, 122.819651 W Map
Discovery NotesPhrodo was found through an enrichment culture of stationary phase B. thuringiensis Kurstaki. Once discovered, it was taken though 4 rounds of purification by process of serial dilution.

Naming NotesThis phage was named Phrodo because while he may be small, he was determined to take the journey to be sequenced and hopefully do big things.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage94
Genome length (bp)164443
GC Content37.8%
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length2404 bp
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC3
Other Cluster Members
Plaque NotesTypical Plaque Morphology: 3mm in diameter mostly turbid with slight outer halo.

HTL Concentration: 4.9 X 10^4 plaque forming units/ micro liter

DNA Concentration: 443.4ng/mL


MorphotypeMyoviridae
Final annotation complete?No
Has been Phamerated?No
Uploaded to GenBank?Yes: KU737347.1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload