Details for Phage RetrieverFever
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| Detailed Information for Phage RetrieverFever | |
| Discovery Information | |
| Found By | Nilusha Jayasinghe and Saba Raza |
| Year Found | 2012 |
| Location Found | Baltimore, MD USA |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| GPS Coordinates | 39.257063, -76.70948 Map |
| Discovery Notes | Phage was extracted from the dark, moist, clumpy soil of the Erickson Courtyard on the campus of the University of Maryland Baltimore County, via direct plating method. Phage was purified via streaking and phage titer. A medium titer lysate (titer: 10^9) and high titer lysate (titer: 10^11) was generated and the DNA was isolated from the HTL. DNA concentration via nanodrap was 433.1 ng/uL. Restriction analysis was conducted and the DNA was cut by Cla1, EcoRI, and HindIII, and the genome size was estimated to be 79 kbp. QC gel showed that the DNA was good quality and possessed little smearing, and had the concentration of 125-250 ng/uL. PCR gel showed one product in the C2 lane. |
| Naming Notes | The phage was named RetrieverFever in honor of the campus from which it was obtained, the UMBC campus (mascot is the retriever). |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Plaque Notes | plaques were cloudy (indicating temperate phage), and when visualized via light microscope, a bulls-eye pattern can be detected in which a clear ring is present on the outside with a cloudier middle. |
| Morphotype | Siphoviridae |
| Final annotation complete? | No |
| Has been Phamerated? | No |
| Uploaded to GenBank? | No |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
