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Details for Phage Stitch
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Detailed Information for Phage Stitch
Discovery Information
Found ByAlexis Brouillette
Year Found2012
Location FoundHarrisonburg, VA United States
Finding InstitutionJames Madison University
Program Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
GPS Coordinates38.42 N, 78.85 W Map
Discovery Notesrocky, grassy field
Naming NotesThis name was inspired by the movie Lilo and Stitch :)
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 24, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage1430
Sanger Finishing Reactions20
Genome length (bp)24321
GC Content30.4%
Character of genome endsCovalently-Bound Terminal Protein
End Determination NotesSanger reads confirmed physical ends of DNA. Similarity to phage phi29 (with well-described covalently-linked terminal proteins) implies terminal proteins.
Fasta file available?Yes: Download fasta file
Plaque NotesStitch had 2 morphologies:
1) Had no halo and was small like a pin point
2) Had a halo and was significantly larger than the other
Final annotation complete?Yes
Has been Phamerated?No
Uploaded to GenBank?Yes: NC_031032.1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload