Details for Phage Stitch
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| Detailed Information for Phage Stitch | |
| Discovery Information | |
| Found By | Alexis Brouillette |
| Year Found | 2012 |
| Location Found | Harrisonburg, VA United States |
| Finding Institution | James Madison University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| GPS Coordinates | 38.42 N, 78.85 W Map |
| Discovery Notes | rocky, grassy field |
| Naming Notes | This name was inspired by the movie Lilo and Stitch :) |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 24, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent Sequencing |
| Approximate Shotgun Coverage | 1430 |
| Sanger Finishing Reactions | 20 |
| Genome length (bp) | 24321 |
| GC Content | 30.4% |
| Character of genome ends | Covalently-Bound Terminal Protein |
| End Determination Notes | Sanger reads confirmed physical ends of DNA. Similarity to phage phi29 (with well-described covalently-linked terminal proteins) implies terminal proteins. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Plaque Notes | Stitch had 2 morphologies: 1) Had no halo and was small like a pin point 2) Had a halo and was significantly larger than the other |
| Morphotype | Podoviridae |
| Final annotation complete? | Yes |
| Has been Phamerated? | No |
| Uploaded to GenBank? | Yes: NC_031032.1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
