Details for Phage OTooleKemple52
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Detailed Information for Phage OTooleKemple52
Discovery Information
Found ByThomas Raymond
Year Found2016
Location FoundChesapeake, VA United States
Finding InstitutionVirginia Commonwealth University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
GPS Coordinates36.696529 N, 76.243623 W Map
Discovery NotesGPS: 36.696529, -76.243623
Physical Description: 4 inches down in soil
Air Temperature: Cloudy, 77 F / 25 C, humid
Collection Date: 8/28/16

First plaque produced on September 6th using an enrichment culture spot test.
Plaque morphology found on September 13th.
HTL produced on October 4th.
Naming NotesNamed using my grandparents' last names and the year they were married (1952)
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 2, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage50
Genome length (bp)161807
GC Content37.9%
Character of genome endsDirect Terminal Repeat
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC2
Other Cluster Members
Plaque NotesPlaques have a pinpoint morphology and grow to about 3 mm in diameter
MorphotypeMyoviridae
Final annotation complete?Yes
Number of Genes298
Number of tRNAs7
Has been Phamerated?No
Uploaded to GenBank?Yes: MF288921
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload