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Details for Phage TsarBomba
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Detailed Information for Phage TsarBomba
Discovery Information
Found ByKevin Liao, Julie Norton
Year Found2014
Location FoundSaratov, Russia
Finding InstitutionUniversity of Maryland, Baltimore County
Program Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
GPS Coordinates51.603333 N, 45.9725 E Map
Discovery NotesSoil (dry/dusty) collected on April 25 2014 at 5:25pm (17:25) from ~5cm beneath a tree (presumed poplar); temperature 54F.
Naming NotesThe name of this phage comes from the Tsar Bomba, the most powerful thermonuclear bomb ever created or detonated. It was named in recognition of its location of discovery in Saratov, Russia. It is also meant to serve as a reminder that it does not take a large bomb to inflict massive damage to countries and peoples, and that efforts against bioterrorism should be cooperative international endeavors, for the good of everyone all over the world.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 17, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage577
Genome length (bp)162486
GC Content40.1%
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length6364 bp
End Determination NotesBuildups of Illumina sequencing reads were clear.
Fasta file available?Yes: Download fasta file
Other Cluster Members
Plaque Notes1 mm turbid plaques on Btk when grown overnight at 30° C. on trypticase soy agar. TEM showed the phage to have an uncontracted tail length of 178.4 nm, capsid width of 84.1 nm, and capsid length of 89.2 nm (n = 20 for all), and a contracted tail length of 96.3 nm (n = 11).
Final annotation complete?Yes
Number of Genes267
Number of tRNAs20
Has been Phamerated?No
Uploaded to GenBank?Yes: KT224359
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
Virtual Digest PictureDownload
EM PictureDownload
DNAMaster FileDownload