Details for Phage Phrodo
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| Detailed Information for Phage Phrodo | |
| Discovery Information | |
| Found By | Shelby Edling |
| Year Found | 2014 |
| Location Found | Belfair, WA United States of America |
| Finding Institution | Virginia Commonwealth University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| GPS Coordinates | 47.46957 N, 122.819651 W Map |
| Discovery Notes | Phrodo was found through an enrichment culture of stationary phase B. thuringiensis Kurstaki. Once discovered, it was taken though 4 rounds of purification by process of serial dilution. |
| Naming Notes | This phage was named Phrodo because while he may be small, he was determined to take the journey to be sequenced and hopefully do big things. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 94 |
| Genome length (bp) | 164443 |
| GC Content | 37.8% |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 2404 bp |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AF |
| Subcluster | AF1 |
| Other Cluster Members |
Click to View |
| Plaque Notes | Typical Plaque Morphology: 3mm in diameter mostly turbid with slight outer halo. HTL Concentration: 4.9 X 10^4 plaque forming units/ micro liter DNA Concentration: 443.4ng/mL |
| Morphotype | Myoviridae |
| Final annotation complete? | Yes |
| Number of Genes | 288 |
| Has been Phamerated? | No |
| Uploaded to GenBank? | Yes: KU737347.1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
