| Detailed Information for Phage Phrodo | |
| Discovery Information | |
| Found By | Shelby Edling |
| Year Found | 2014 |
| Location Found | Belfair, WA United States of America |
| Finding Institution | Virginia Commonwealth University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| GPS Coordinates | 47.46957 N, 122.819651 W Map |
| Discovery Notes | Phrodo was found through an enrichment culture of stationary phase B. thuringiensis Kurstaki. Once discovered, it was taken though 4 rounds of purification by process of serial dilution. |
| Naming Notes | This phage was named Phrodo because while he may be small, he was determined to take the journey to be sequenced and hopefully do big things. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 94 |
| Genome length (bp) | 164443 |
| GC Content | 37.8% |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 2404 bp |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | C |
| Subcluster | C3 |
| Other Cluster Members |
Click to ViewHydralpha (None) JoeB (None) AaronPhadgers (C1) ALPS (C1) Archie14 (C1) Azalea (C1) Belinda (C1) Bhoosica (C1) Cletus (C1) DingnCase (C1) Dingus (C1) DirtyBetty (C1) Doofinshmirtz (C1) Elmer (C1) Escavirius (C1) Eyuki (C1) Freefood (C1) FreightTrain (C1) Grumio (C1) Hakuna (C1) Helga (C1) HonestAbe (C1) Ilona (C1) Kida (C1) Kota (C1) Lilihipha (C1) Megatron (C1) MightyMouse (C1) Momo (C1) Nemo (C1) Nigalana (C1) NotTheCreek (C1) OmnioDeoprimus (C1) Parsons (C1) PPIsBest (C1) Rex16 (C1) SageFayge (C1) SalinJah (C1) STSebastian (C1) Terra (C1) WPh (C1) Zuko (C1) Anthony (C2) Bastille (C2) CAM003 (C2) Evoli (C2) Herbert (C2) HoodyT (C2) Hudslayer (C2) Janet (C2) Kamfam (C2) OTooleKemple52 (C2) Vinny (C2) Adelynn (C3) Ampersand (C3) Anthos (C3) AvesoBmore (C3) B4 (C3) BigBertha (C3) ChannelFever (C3) Chotacabras (C3) ChurroBlast (C3) Flapjack (C3) GypsyDanger (C3) Joonam (C3) Juglone (C3) Natp (C3) Oregano (C3) Phabio (C3) Phrodo (C3) Riley (C3) Spock (C3) Taffo16 (C3) Troll (C3) Typhen (C3) BC4U (C4) BCP78 (C4) TsarBomba (C4) Bcp1 (C5) BM10 (C5) Hobo (C5) Kioshi (C5) |
| Plaque Notes | Typical Plaque Morphology: 3mm in diameter mostly turbid with slight outer halo. HTL Concentration: 4.9 X 10^4 plaque forming units/ micro liter DNA Concentration: 443.4ng/mL |
| Morphotype | Myoviridae |
| Final annotation complete? | Yes |
| Number of Genes | 288 |
| Has been Phamerated? | No |
| Uploaded to GenBank? | Yes: KU737347.1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
