Details for Phage Troll
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Detailed Information for Phage Troll
Discovery Information
Found BySabrina Mathew & Dongha Le
Year Found2012
Location FoundBurtonsville, MD USA
Finding InstitutionUniversity of Maryland, Baltimore County
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
GPS Coordinates39.111219 N, 76.932475 W Map
Discovery NotesThis BTK bacteriophage was isolated from a soil sample taken from an urban housing area in Burtonsville on 9/9/12.
Naming NotesThe name "Troll" was chosen due to the resemblance of the phage’s head to that of the hair of a Troll doll.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 24, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage310
Sanger Finishing Reactions9
Genome length (bp)163019
GC Content37.8%
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length2711 bp
End Determination NotesBy doubling of read coverage in Consed.
Fasta file available?Yes: Download fasta file
Characterization
ClusterAF
SubclusterAF1
Other Cluster Members
Plaque NotesTroll creates plaques that are oval shaped and turbid suggesting that Troll is a temperate phage. The plaques are around 3 mm or smaller in diameter.
MorphotypeMyoviridae
Final annotation complete?Yes
Number of Genes289
Has been Phamerated?No
Uploaded to GenBank?Yes: KF208639.2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
Virtual Digest PictureDownload
EM PictureDownload
DNAMaster FileDownload