Details for Phage TsarBomba
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| Detailed Information for Phage TsarBomba | |
| Discovery Information | |
| Found By | Kevin Liao, Julie Norton |
| Year Found | 2014 |
| Location Found | Saratov, Russia |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| GPS Coordinates | 51.603333 N, 45.9725 E Map |
| Discovery Notes | Soil (dry/dusty) collected on April 25 2014 at 5:25pm (17:25) from ~5cm beneath a tree (presumed poplar); temperature 54F. |
| Naming Notes | The name of this phage comes from the Tsar Bomba, the most powerful thermonuclear bomb ever created or detonated. It was named in recognition of its location of discovery in Saratov, Russia. It is also meant to serve as a reminder that it does not take a large bomb to inflict massive damage to countries and peoples, and that efforts against bioterrorism should be cooperative international endeavors, for the good of everyone all over the world. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 17, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 577 |
| Genome length (bp) | 162486 |
| GC Content | 40.1% |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 6364 bp |
| End Determination Notes | Buildups of Illumina sequencing reads were clear. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AF |
| Subcluster | AF4 |
| Other Cluster Members |
Click to View |
| Plaque Notes | 1 mm turbid plaques on Btk when grown overnight at 30° C. on trypticase soy agar. TEM showed the phage to have an uncontracted tail length of 178.4 nm, capsid width of 84.1 nm, and capsid length of 89.2 nm (n = 20 for all), and a contracted tail length of 96.3 nm (n = 11). |
| Morphotype | Myoviridae |
| Final annotation complete? | Yes |
| Number of Genes | 267 |
| Number of tRNAs | 20 |
| Has been Phamerated? | No |
| Uploaded to GenBank? | Yes: KT224359 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| DNAMaster File | Download |
