Details for Phage NotTheCreek
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| Detailed Information for Phage NotTheCreek | |
| Discovery Information | |
| Found By | Natasha Smith |
| Year Found | 2013 |
| Location Found | South Brunswick, NJ United States |
| Finding Institution | Virginia Commonwealth University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| GPS Coordinates | 40.399106 N, 74.51575 W Map |
| Discovery Notes | The phage was isolated from soil found at the bottom of a 6 in hole in the front yard of a house in a residential neighborhood. It was approximately 24 degrees Celsius. |
| Naming Notes | I was originally trying to isolate a phage from a soil sample at the edge of a creek, but wasn't able to. "NotTheCreek" is short for "not from the creek." |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 5, 2014 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Approximate Shotgun Coverage | 100 |
| Sanger Finishing Reactions | 6 |
| Genome length (bp) | 159336 |
| GC Content | 38.2% |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 2585 bp |
| End Determination Notes | By doubling of read coverage in Consed. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | S |
| Subcluster | -- |
| Other Cluster Members | |
| Plaque Notes | Two consistent plaque morphologies are present, 1mm in diameter and .6mm respectively. Plaques are clear and without halos. |
| Morphotype | Myoviridae |
| Final annotation complete? | Yes |
| Number of Genes | 296 |
| Has been Phamerated? | No |
| Uploaded to GenBank? | Yes: KU737351.1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
