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Details for Phage GypsyDanger
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Detailed Information for Phage GypsyDanger
Discovery Information
Found ByAhmed Farshori, William Blankenship
Year Found2013
Location FoundBaltimore, MD United States
Finding InstitutionUniversity of Maryland, Baltimore County
Program Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
GPS Coordinates39.25455 N, 76.703453 W Map
Discovery NotesThe phage GypsyDanger was discovered in soil sample from a stream running across the periphery of the University of Maryland Baltimore County campus. Soil was extracted from approximately 6cm below the damp stream bank very close to the impervious surface of a parking lot.
Naming NotesThe name GypsyDanger was chosen because it is something easy and fun to relate to for the students and professors this year.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 13, 2014
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3187
Genome length (bp)164029
GC Content37.6%
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length2293 bp
End Determination NotesBuildup of Illumina sequencing reads was clear.
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC3
Other Cluster Members
Plaque NotesGypsyDanger forms standard lytic plaques with one unique feature; if the plaques are incubated longer than 24 hours the larger ones will form distinct haloes around them that all skew in a single direction, resulting in a "falling meteor" shape. These plaques have been reproduced multiple times and are an anomaly, as other phages in the same lab do not produce such distinct, unidirectionally skewed plaques.
MorphotypeMyoviridae
Final annotation complete?No
Has been Phamerated?No
Uploaded to GenBank?No
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload