| Detailed Information for Phage Nigalana | |
| Discovery Information | |
| Found By | Navpreet Saini |
| Year Found | 2013 |
| Location Found | Manassas, VA United States of America |
| Finding Institution | Virginia Commonwealth University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| GPS Coordinates | 38.742153 N, 77.426758 W Map |
| Discovery Notes | The BTK bacteriophage "Nigalana" was isolated from a soil sample taken from a rural area in Manassas on 9/3/13. The area was known for having pasture animals and the temperature was around 21°C. The dirt sample was enriched in the VCU phage discovery lab. |
| Naming Notes | The name Nigalana was given to the bacteriophage because "Nigalana" by definition in Punjabi means "to devour" and in Greek "Phagein" means "to devour". |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 22, 2014 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Approximate Shotgun Coverage | 96 |
| Sanger Finishing Reactions | 33 |
| Genome length (bp) | 163041 |
| GC Content | 38.7% |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 2867 bp |
| End Determination Notes | Buildups of reads in the 454 sequencing data. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | C |
| Subcluster | C1 |
| Other Cluster Members |
Click to ViewHydralpha (None) JoeB (None) AaronPhadgers (C1) ALPS (C1) Archie14 (C1) Azalea (C1) Belinda (C1) Bhoosica (C1) Cletus (C1) DingnCase (C1) Dingus (C1) DirtyBetty (C1) Doofinshmirtz (C1) Elmer (C1) Escavirius (C1) Eyuki (C1) Freefood (C1) FreightTrain (C1) Grumio (C1) Hakuna (C1) Helga (C1) HonestAbe (C1) Ilona (C1) Kida (C1) Kota (C1) Lilihipha (C1) Megatron (C1) MightyMouse (C1) Momo (C1) Nemo (C1) Nigalana (C1) NotTheCreek (C1) OmnioDeoprimus (C1) Parsons (C1) PPIsBest (C1) Rex16 (C1) SageFayge (C1) SalinJah (C1) STSebastian (C1) Terra (C1) WPh (C1) Zuko (C1) Anthony (C2) Bastille (C2) CAM003 (C2) Evoli (C2) Herbert (C2) HoodyT (C2) Hudslayer (C2) Janet (C2) Kamfam (C2) OTooleKemple52 (C2) Vinny (C2) Adelynn (C3) Ampersand (C3) Anthos (C3) AvesoBmore (C3) B4 (C3) BigBertha (C3) ChannelFever (C3) Chotacabras (C3) ChurroBlast (C3) Flapjack (C3) GypsyDanger (C3) Joonam (C3) Juglone (C3) Natp (C3) Oregano (C3) Phabio (C3) Phrodo (C3) Riley (C3) Spock (C3) Taffo16 (C3) Troll (C3) Typhen (C3) BC4U (C4) BCP78 (C4) TsarBomba (C4) Bcp1 (C5) BM10 (C5) Hobo (C5) Kioshi (C5) |
| Plaque Notes | The plates were shown to have clear plaque spots and they contained no halos. Each plaque had an average diameter of 2 mm. |
| Morphotype | Myoviridae |
| Final annotation complete? | Yes |
| Number of Genes | 302 |
| Has been Phamerated? | No |
| Uploaded to GenBank? | Yes: KU737344.1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
