Details for Phage Nigalana
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| Detailed Information for Phage Nigalana | |
| Discovery Information | |
| Found By | Navpreet Saini |
| Year Found | 2013 |
| Location Found | Manassas, VA United States of America |
| Finding Institution | Virginia Commonwealth University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| GPS Coordinates | 38.742153 N, 77.426758 W Map |
| Discovery Notes | The BTK bacteriophage "Nigalana" was isolated from a soil sample taken from a rural area in Manassas on 9/3/13. The area was known for having pasture animals and the temperature was around 21°C. The dirt sample was enriched in the VCU phage discovery lab. |
| Naming Notes | The name Nigalana was given to the bacteriophage because "Nigalana" by definition in Punjabi means "to devour" and in Greek "Phagein" means "to devour". |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 22, 2014 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Approximate Shotgun Coverage | 96 |
| Sanger Finishing Reactions | 33 |
| Genome length (bp) | 163041 |
| GC Content | 38.7% |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 2867 bp |
| End Determination Notes | Buildups of reads in the 454 sequencing data. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | S |
| Subcluster | -- |
| Other Cluster Members | |
| Plaque Notes | The plates were shown to have clear plaque spots and they contained no halos. Each plaque had an average diameter of 2 mm. |
| Morphotype | Myoviridae |
| Final annotation complete? | Yes |
| Number of Genes | 302 |
| Has been Phamerated? | No |
| Uploaded to GenBank? | Yes: KU737344.1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
